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p3xFLAG-CMV-Drosha (E419)
(Plasmid #171534)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171534 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3xFLAG-CMV 7.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DROSHA
  • Species
    H. sapiens (human)
  • Mutation
    a Drosha mutant stopping at E419
  • Entrez Gene
    DROSHA (a.k.a. ETOHI2, HSA242976, RANSE3L, RN3, RNASE3L, RNASEN)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note DROSHA contains 4 mutations-S30P, A135V, S201G and S321L. These do not affect function as they are found in the original pCK-Drosha-FLAG (Lee et al, 2003, Nature).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3xFLAG-CMV-Drosha (E419) was a gift from Yan Zeng (Addgene plasmid # 171534 ; http://n2t.net/addgene:171534 ; RRID:Addgene_171534)

  • For your References section:

    The insertion in the double-stranded RNA binding domain of human Drosha is important for its function. Zhang X, Li P, Lin J, Huang H, Yin B, Zeng Y. Biochim Biophys Acta Gene Regul Mech. 2017 Dec;1860(12):1179-1188. doi: 10.1016/j.bbagrm.2017.10.004. Epub 2017 Nov 3. 10.1016/j.bbagrm.2017.10.004 PubMed 29109067
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