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M15 pCS-twhh-nBeta gal
(Plasmid #17152)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17152 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS-NBeta gal

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    twhh promoter
  • Alt name
    tiggy-winkle hedgehog
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    5200
  • Entrez Gene
    shhb (a.k.a. twh, twhh)
  • Tag / Fusion Protein
    • nuclear beta-gal (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (unknown if destroyed)
  • 3′ cloning site HindIII (destroyed during cloning)
  • 5′ sequencing primer NA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: twhh promoter (5.2 Kb), nuclear Beta gal reporter (3 Kb)

Plasmid History:
Source: zebrafish.

Notes for Use:
Gives notochord-specific expression of Beta-gal.
Citation: Du et al., 1997, J. Cell Biology.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M15 pCS-twhh-nBeta gal was a gift from Randall Moon (Addgene plasmid # 17152 ; http://n2t.net/addgene:17152 ; RRID:Addgene_17152)

  • For your References section:

    Positive and negative regulation of muscle cell identity by members of the hedgehog and TGF-beta gene families. Du SJ, Devoto SH, Westerfield M, Moon RT. J Cell Biol. 1997 Oct 6. 139(1):145-56. 10.1083/jcb.139.1.145 PubMed 9314535
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