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Addgene

pX330A-Prdm14-L4-#2
(Plasmid #171512)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171512 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    px330A
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Prdm14
  • gRNA/shRNA sequence
    GGTCTATCTGGCTCCGTTGC
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Prdm14

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX330A-Prdm14-L4-#2 was a gift from Hans Schöler (Addgene plasmid # 171512 ; http://n2t.net/addgene:171512 ; RRID:Addgene_171512)

  • For your References section:

    Residual pluripotency is required for inductive germ cell segregation. Aramaki S, Kagiwada S, Wu G, Obridge D, Adachi K, Kutejova E, Lickert H, Hubner K, Scholer HR. EMBO Rep. 2021 Aug 4;22(8):e52553. doi: 10.15252/embr.202152553. Epub 2021 Jun 22. 10.15252/embr.202152553 PubMed 34156139
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