pJC5-4-insulator-BREreporter-insulator
(Plasmid #171499)

• Purpose: injections to make Bmp reporter mouse transgenic line
• Depositing Lab: James Briscoe
• Publication: Ivanovitch et al PLoS Biol. 2021 May 17;19(5):e3001200. doi: 10.1371/journal.pbio.3001200. eCollection 2021 May.

Backbone

• Vector backbone: pGEM47
• Backbone size w/o insert (bp): 2750
• Total vector size (bp): 3950
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Insulator BRE-MLP:H2B-Turqoise
• Species: M. musculus (mouse), Synthetic
• Insert Size (bp): 6890
• Promoter: BRE-MLP

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: cactcattaggcaccccag

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene QC NGS analysis was unable to generate a full plasmid sequence. The depositing laboratory has indicated that these sequence discrepancies should not be of any consequence to function. Please visit https://www.biorxiv.org/content/10.1101/2020.07.12.198994v2 for bioRxiv preprint.