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Addgene

M11 Cardiac Actin Promoter pCarA
(Plasmid #17148)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17148 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCSKA
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Xenopus expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cardiac actin promoter
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    3270
  • GenBank ID
    X04669
  • Entrez Gene
    actc1 (a.k.a. actc, acta1, MGC52636, MGC79039)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid History:
Constructed by Enrique Amaya.

Note for Use:
Constructed by cloning the 3270bp KpnI-SalI fragment from DNA#254 (from Tim Mohun) into KpnI-SalI digested pCSKA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M11 Cardiac Actin Promoter pCarA was a gift from Randall Moon (Addgene plasmid # 17148 ; http://n2t.net/addgene:17148 ; RRID:Addgene_17148)
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