M10 NCAM Promoter
(Plasmid #17147)

• Depositing Lab: Randall Moon
• Publication: Other Plasmids (unpublished)

Backbone

• Vector backbone: pBluescript KS(+)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: Xenopus expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NCAM promoter
• Alt name: NCAM
• Species: X. laevis (frog)
• Insert Size (bp): 1300
• Entrez Gene: ncam1-b (a.k.a. ncam, n-cam, ncam1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SmaI/DraI (destroyed during cloning)
• 3′ cloning site: SmaI/DraI (destroyed during cloning)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid History:
Constructed Paul A. Kreig, July.

Note for Use:

This construction contains a 1.3 Kb Dra I fragment of the NCAM promoter and should be okay for general neural expression. The fragment is cloned into the Sma I site of Bluescript KS+. The NCAM promoter does not contain a TATA box and transcription starts at numerous sites over about 350 bases. All of the potential start sites are contained in this 1.3 Kb fragment, which ends in the 5' UTR of the NCAM gene. The Dra I fragment starts at the very beginning of the GenBank sequence (starting with aaa) and ends at base 1358 of the GenBank sequence.