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mChF-Cav1s
(Plasmid #171464)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171464 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry-C1 (modified)
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 6176
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry-FKBP12-Caveolin1(61-178 aa)
  • Alt name
    mChF-Cav1s
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1476
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CGGTGGGAGGTC
  • 3′ sequencing primer CCTCTACAAATGTGG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: The plasmid contains a D220E mutation in mCherry compared to the depositor's sequence. This mutation does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mChF-Cav1s was a gift from Takafumi Miyamoto (Addgene plasmid # 171464 ; http://n2t.net/addgene:171464 ; RRID:Addgene_171464)

  • For your References section:

    Rapid manipulation of mitochondrial morphology in a living cell with iCMM. Miyamoto T, Uosaki H, Mizunoe Y, Han SI, Goto S, Yamanaka D, Masuda M, Yoneyama Y, Nakamura H, Hattori N, Takeuchi Y, Ohno H, Sekiya M, Matsuzaka T, Hakuno F, Takahashi SI, Yahagi N, Ito K, Shimano H. Cell Rep Methods. 2021 Jul 22;1(4):100052. doi: 10.1016/j.crmeth.2021.100052. eCollection 2021 Aug 23. 10.1016/j.crmeth.2021.100052 PubMed 35475143
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