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mActZ-FY
(Plasmid #171463)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171463 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEYFP-C1 (modified)
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 7697
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ActA(30-262 aa)-Zyxin(2-380 aa)-FKBP12-EYFP
  • Alt name
    mActZ-FY
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2997
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CGGTGGGAGGTC
  • 3′ sequencing primer CCTCTACAAATGTGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mActZ-FY was a gift from Takafumi Miyamoto (Addgene plasmid # 171463 ; http://n2t.net/addgene:171463 ; RRID:Addgene_171463)

  • For your References section:

    Rapid manipulation of mitochondrial morphology in a living cell with iCMM. Miyamoto T, Uosaki H, Mizunoe Y, Han SI, Goto S, Yamanaka D, Masuda M, Yoneyama Y, Nakamura H, Hattori N, Takeuchi Y, Ohno H, Sekiya M, Matsuzaka T, Hakuno F, Takahashi SI, Yahagi N, Ito K, Shimano H. Cell Rep Methods. 2021 Jul 22;1(4):100052. doi: 10.1016/j.crmeth.2021.100052. eCollection 2021 Aug 23. 10.1016/j.crmeth.2021.100052 PubMed 35475143
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