YF-Cav1s
(Plasmid
#171462)
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PurposeExpresses YF-Cav1s, a FiCE of iCMM, in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 171462 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEYFP-C1 (modified)
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 6185
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEYFP-FKBP12-Caveolin1(61-178 aa)
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Alt nameYF-Cav1s
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SpeciesH. sapiens (human)
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Insert Size (bp)1485
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer CGGTGGGAGGTC
- 3′ sequencing primer CCTCTACAAATGTGG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YF-Cav1s was a gift from Takafumi Miyamoto (Addgene plasmid # 171462 ; http://n2t.net/addgene:171462 ; RRID:Addgene_171462) -
For your References section:
Rapid manipulation of mitochondrial morphology in a living cell with iCMM. Miyamoto T, Uosaki H, Mizunoe Y, Han SI, Goto S, Yamanaka D, Masuda M, Yoneyama Y, Nakamura H, Hattori N, Takeuchi Y, Ohno H, Sekiya M, Matsuzaka T, Hakuno F, Takahashi SI, Yahagi N, Ito K, Shimano H. Cell Rep Methods. 2021 Jul 22;1(4):100052. doi: 10.1016/j.crmeth.2021.100052. eCollection 2021 Aug 23. 10.1016/j.crmeth.2021.100052 PubMed 35475143