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Addgene

Tom20-CR
(Plasmid #171461)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171461 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pECFP-C1 (modified)
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5897
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tom20 (1-34 aa)-ECFP-FRB (2021-2113 aa)
  • Alt name
    Tom20-CR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1197
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CGGTGGGAGGTC
  • 3′ sequencing primer CCTCTACAAATGTGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tom20-CR was a gift from Takafumi Miyamoto (Addgene plasmid # 171461 ; http://n2t.net/addgene:171461 ; RRID:Addgene_171461)

  • For your References section:

    Rapid manipulation of mitochondrial morphology in a living cell with iCMM. Miyamoto T, Uosaki H, Mizunoe Y, Han SI, Goto S, Yamanaka D, Masuda M, Yoneyama Y, Nakamura H, Hattori N, Takeuchi Y, Ohno H, Sekiya M, Matsuzaka T, Hakuno F, Takahashi SI, Yahagi N, Ito K, Shimano H. Cell Rep Methods. 2021 Jul 22;1(4):100052. doi: 10.1016/j.crmeth.2021.100052. eCollection 2021 Aug 23. 10.1016/j.crmeth.2021.100052 PubMed 35475143
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