3.8NBetaT-CAT
(Plasmid
#17146)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17146 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBLCAT3
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Backbone manufacturerATCC
- Backbone size w/o insert (bp) 4300
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBeta-tubulin promoter
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SpeciesX. laevis (frog)
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Insert Size (bp)3800
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GenBank IDEF989124
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Tag
/ Fusion Protein
- CAT (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer M13 forward 20 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Constructed Rebecca Beach and Paul A. Kreig.
3.8Kb fragment of the Xenopus neural Beta-tubulin promoter inserted (in several cloning steps) into the CAT expression vector pBLCAT3. The 5' end of the neural Beta-tubulin sequence is defined by the natural Xba1 site that occurs in the genomic DNA. The 3' end of the insert is produced by PCR primer located 8bp upstream of hte initiation ATP and 120bp downstream of the transcription start site. The neural Beta-tubulin sequences may be excised as a Hind3 fragment.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
3.8NBetaT-CAT was a gift from Paul Krieg (Addgene plasmid # 17146 ; http://n2t.net/addgene:17146 ; RRID:Addgene_17146)
Map uploaded by the depositor.
