pcDNA3.1(+)-HaloTag N58-(GGS)X6-FRB
(Plasmid
#171427)
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PurposeExpression of HaloTag N58-(GGS)X6-FRB in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 171427 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHaloTag N58-(GGS)X6-FRB
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1(+)-HaloTag N58-(GGS)X6-FRB was a gift from Yujie Sun (Addgene plasmid # 171427 ; http://n2t.net/addgene:171427 ; RRID:Addgene_171427) -
For your References section:
TagBiFC technique allows long-term single-molecule tracking of protein-protein interactions in living cells. Shao S, Zhang H, Zeng Y, Li Y, Sun C, Sun Y. Commun Biol. 2021 Mar 19;4(1):378. doi: 10.1038/s42003-021-01896-7. 10.1038/s42003-021-01896-7 PubMed 33742089