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Addgene

GST-OPTNS177DS473D
(Plasmid #171425)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171425 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGST2
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OPTN
  • Species
    H. sapiens (human)
  • Mutation
    phosphorylation mutant (S177D, S473D)
  • Entrez Gene
    OPTN (a.k.a. ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-OPTNS177DS473D was a gift from James Hurley (Addgene plasmid # 171425 ; http://n2t.net/addgene:171425 ; RRID:Addgene_171425)

  • For your References section:

    Reconstitution of cargo-induced LC3 lipidation in mammalian selective autophagy. Chang C, Shi X, Jensen LE, Yokom AL, Fracchiolla D, Martens S, Hurley JH. Sci Adv. 2021 Apr 23;7(17). pii: 7/17/eabg4922. doi: 10.1126/sciadv.abg4922. Print 2021 Apr. 10.1126/sciadv.abg4922 PubMed 33893090
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