WIPI2d-TEVcs-TSF
(Plasmid
#171419)
-
PurposeFor the purifcation of WIPI2d protein
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 171419 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCAG
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameWIPI2d
-
SpeciesH. sapiens (human)
-
Entrez GeneWIPI2 (a.k.a. ATG18B, Atg21, CGI-50, IDDSSA, WIPI-2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
-
Supplemental Documents
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
WIPI2d-TEVcs-TSF was a gift from James Hurley (Addgene plasmid # 171419 ; http://n2t.net/addgene:171419 ; RRID:Addgene_171419) -
For your References section:
Reconstitution of cargo-induced LC3 lipidation in mammalian selective autophagy. Chang C, Shi X, Jensen LE, Yokom AL, Fracchiolla D, Martens S, Hurley JH. Sci Adv. 2021 Apr 23;7(17). pii: 7/17/eabg4922. doi: 10.1126/sciadv.abg4922. Print 2021 Apr. 10.1126/sciadv.abg4922 PubMed 33893090