GST-TEVcs- FIP200 delta641-779-MBP
(Plasmid
#171411)
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PurposeFor the purifcation of human ULK1 truncated complex
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 171411 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFIP200
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SpeciesSynthetic
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Mutationtruncation (delta641-779-MBP)
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Entrez GeneRB1CC1 (a.k.a. ATG17, CC1, FIP200, PPP1R131)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GST-TEVcs- FIP200 delta641-779-MBP was a gift from James Hurley (Addgene plasmid # 171411 ; http://n2t.net/addgene:171411 ; RRID:Addgene_171411) -
For your References section:
Reconstitution of cargo-induced LC3 lipidation in mammalian selective autophagy. Chang C, Shi X, Jensen LE, Yokom AL, Fracchiolla D, Martens S, Hurley JH. Sci Adv. 2021 Apr 23;7(17). pii: 7/17/eabg4922. doi: 10.1126/sciadv.abg4922. Print 2021 Apr. 10.1126/sciadv.abg4922 PubMed 33893090