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CMV-RfxCas13d-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA
(Plasmid #171380)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171380 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCX539
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    humanized RfxCas13d
  • Alt name
    RfxCas13d
  • Alt name
    pCX850
  • Species
    Synthetic
  • Promoter CMV, U6
  • Tag / Fusion Protein
    • HA (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-RfxCas13d-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA was a gift from Hui Yang (Addgene plasmid # 171380 ; http://n2t.net/addgene:171380 ; RRID:Addgene_171380)

  • For your References section:

    Programmable RNA editing with compact CRISPR-Cas13 systems from uncultivated microbes. Xu C, Zhou Y, Xiao Q, He B, Geng G, Wang Z, Cao B, Dong X, Bai W, Wang Y, Wang X, Zhou D, Yuan T, Huo X, Lai J, Yang H. Nat Methods. 2021 May;18(5):499-506. doi: 10.1038/s41592-021-01124-4. Epub 2021 May 3. 10.1038/s41592-021-01124-4 PubMed 33941935
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