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pYeDP60-hATP13A2-E343A
(Plasmid #171378)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171378 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYeDP60
  • Backbone manufacturer
    Joseph Lyons
  • Backbone size w/o insert (bp) 9259
  • Total vector size (bp) 13090
  • Modifications to backbone
    insert of hATP13A2, thrombin cleavage site, BAD tag
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human ATP13A2 variant 2 wild-type
  • Alt name
    hATP13A2
  • Alt name
    polyamine-transporting ATPase 13A2 isoform 2 [Homo sapiens]
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3830
  • Mutation
    E343A
  • GenBank ID
    NM_001141973.3
  • Promoter URA3
  • Tag / Fusion Protein
    • BAD tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site ClaI (unknown if destroyed)
  • 5′ sequencing primer TAACTTGGCCCACGCTGAAA
  • 3′ sequencing primer CATGTTGGAATCTGTTCTTGATCAATGCCTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYeDP60-hATP13A2-E343A was a gift from Peter Vangheluwe (Addgene plasmid # 171378 ; http://n2t.net/addgene:171378 ; RRID:Addgene_171378)

  • For your References section:

    ATP13A2 deficiency disrupts lysosomal polyamine export. van Veen S, Martin S, Van den Haute C, Benoy V, Lyons J, Vanhoutte R, Kahler JP, Decuypere JP, Gelders G, Lambie E, Zielich J, Swinnen JV, Annaert W, Agostinis P, Ghesquiere B, Verhelst S, Baekelandt V, Eggermont J, Vangheluwe P. Nature. 2020 Feb;578(7795):419-424. doi: 10.1038/s41586-020-1968-7. Epub 2020 Jan 29. 10.1038/s41586-020-1968-7 PubMed 31996848
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