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pcDNA3.1(-)-Gαi3-YFP (Citrine variant, b/c loop insert)
(Plasmid #171375)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171375 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(-)
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 7216
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gai3-YFP
  • Species
    R. norvegicus (rat), Synthetic
  • Insert Size (bp)
    1788
  • Promoter CMV
  • Tag / Fusion Protein
    • YFP

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(-)-Gαi3-YFP (Citrine variant, b/c loop insert) was a gift from Mikel Garcia-Marcos (Addgene plasmid # 171375 ; http://n2t.net/addgene:171375 ; RRID:Addgene_171375)

  • For your References section:

    Dominant-negative Galpha subunits are a mechanism of dysregulated heterotrimeric G protein signaling in human disease. Marivin A, Leyme A, Parag-Sharma K, DiGiacomo V, Cheung AY, Nguyen LT, Dominguez I, Garcia-Marcos M. Sci Signal. 2016 Apr 12;9(423):ra37. doi: 10.1126/scisignal.aad2429. 10.1126/scisignal.aad2429 PubMed 27072656
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