Skip to main content
Addgene

pcDNA3.1-Gα13(EE)-YFP
(Plasmid #171374)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171374 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3.1+
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 7300
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ga13(EE)-YFP
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1878
  • Promoter CMV
  • Tag / Fusion Protein
    • Glu-Glu

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Generated by first introducing a SacII restriction site after amino acid P131 into pcDNA3.1-Gα13(EE) [cDNA Resource Center, Bloomsburg University] and then inserting Citrine, flanked by a GSG linker on each end, at this site by Gibson assembly

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-Gα13(EE)-YFP was a gift from Mikel Garcia-Marcos (Addgene plasmid # 171374 ; http://n2t.net/addgene:171374 ; RRID:Addgene_171374)

  • For your References section:

    Revealing the Activity of Trimeric G-proteins in Live Cells with a Versatile Biosensor Design. Maziarz M, Park JC, Leyme A, Marivin A, Garcia-Lopez A, Patel PP, Garcia-Marcos M. Cell. 2020 Jun 27. pii: S0092-8674(20)30752-2. doi: 10.1016/j.cell.2020.06.020. 10.1016/j.cell.2020.06.020 PubMed 32634377
Related items: