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Addgene

pX459-SpCas9-NG
(Plasmid #171370)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171370 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pX459
  • Backbone size (bp) 9175
  • Modifications to backbone
    pX459-SpCas9-NG was constructed by replacing the Cas9 coding sequence of pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene plasmid #62988) with that of pX330-SpCas9-NG (Addgene plasmid #117919) using AgeI (R0552, NEB, MA, USA) and FseI (R0588, NEB) restriction enzymes.
  • Vector type
    Mammalian Expression, CRISPR
  • Promoter U6 for sgRNA; CBh for Cas9-2A-puroR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX459-SpCas9-NG was a gift from Masahito Ikawa (Addgene plasmid # 171370 ; http://n2t.net/addgene:171370 ; RRID:Addgene_171370)

  • For your References section:

    Precise CAG repeat contraction in a Huntington's Disease mouse model is enabled by gene editing with SpCas9-NG. Oura S, Noda T, Morimura N, Hitoshi S, Nishimasu H, Nagai Y, Nureki O, Ikawa M. Commun Biol. 2021 Jun 23;4(1):771. doi: 10.1038/s42003-021-02304-w. 10.1038/s42003-021-02304-w PubMed 34163001
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