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Flag-neo-PP1-Alpha-H248K
(Plasmid #171268)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171268 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3XFLAG-CMV-10
  • Backbone manufacturer
    Sigma-Aldrich
  • Backbone size w/o insert (bp) 6400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant
  • Alt name
    PP1-Alpha-H248K
  • Alt name
    PPP1CA-H248K
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Mutation
    PPP1CA-His248Lys (H248K)
  • Entrez Gene
    PPP1CA (a.k.a. PP-1A, PP1A, PP1alpha, PPP1A)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer CMV_fwd_primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The construction is based on PCR amplification using Pfu DNA polymerase and DNA mutagenesis.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-neo-PP1-Alpha-H248K was a gift from Steven Balk (Addgene plasmid # 171268 ; http://n2t.net/addgene:171268 ; RRID:Addgene_171268)

  • For your References section:

    Protein phosphatase 1 suppresses androgen receptor ubiquitylation and degradation. Liu X, Han W, Gulla S, Simon NI, Gao Y, Cai C, Yang H, Zhang X, Liu J, Balk SP, Chen S. Oncotarget. 2016 Jan 12;7(2):1754-64. doi: 10.18632/oncotarget.6434. 10.18632/oncotarget.6434 PubMed 26636645
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