Flag-M4-AR-K313A-K318A
(Plasmid #171260)

• Purpose: Mammalian expression of 3xFlag-tagged human androgen receptor Lysine mutant: AR-Lys313Ala-Lys318Ala (AR-K313A-K318A)
• Depositing Lab: Steven Balk
• Publication: Balk lab plasmids (unpublished)

Backbone

• Vector backbone: p3XFLAG-CMV-10
• Backbone manufacturer: Sigma-Aldrich
• Backbone size w/o insert (bp): 6400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3xFlag-tagged human androgen receptor Lys313Ala-Lys318Ala mutant
• Alt name: Flag-AR-K313/318A
• Species: H. sapiens (human)
• Insert Size (bp): 3000
• Mutation: AR-Lys313Ala-Lys318Ala (AR-K313A-K318A)
• Entrez Gene: AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: CMV_fwd_primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Flag-tagged human AR Lysine mutant, and the exact amino position and composition may slightly vary based on human AR versions. Two XmaI sites on the vector backbone and one EcoRI site at the 3'UTR of AR cDNA were deleted by DNA mutagenesis.