Flag-M4-AR-W741C-S81D
(Plasmid #171249)

• Purpose: Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Asp (AR-W741C-S81D)
• Depositing Lab: Steven Balk
• Publication: Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83. doi: 10.1074/jbc.M111.325290. Epub 2012 Jan 24.

Backbone

• Vector backbone: p3XFLAG-CMV-10
• Backbone manufacturer: Sigma-Aldrich
• Backbone size w/o insert (bp): 6400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3xFlag-tagged human androgen receptor Trp741Cys-Ser81Asp mutant
• Alt name: Flag-AR-W741C-S81D
• Species: H. sapiens (human)
• Insert Size (bp): 3000
• Mutation: AR-Trp741Cys-Ser81Asp (AR-W741C-S81D)
• Entrez Gene: AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: CMV_fwd_primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Flag-tagged human AR Ser-Pro phosphorylation mutant that binds to bicalutamide, and the exact amino position and composition may slightly vary based on human AR versions.Two XmaI sites on the vector backbone and one EcoRI site at the 3'UTR of AR cDNA were deleted by DNA mutagenesis.