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Addgene

HA-AR-LBD
(Plasmid #171239)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171239 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)-kozakHAHA
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    1xHA-tagged human androgen receptor trancate:Ligand-binding domain
  • Alt name
    HA-AR-LBD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    800
  • Mutation
    AR truncate
  • Entrez Gene
    AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CMV_fwd_primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

It is based on a single HA tagged vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-AR-LBD was a gift from Steven Balk (Addgene plasmid # 171239 ; http://n2t.net/addgene:171239 ; RRID:Addgene_171239)

  • For your References section:

    Protein phosphatase 1 suppresses androgen receptor ubiquitylation and degradation. Liu X, Han W, Gulla S, Simon NI, Gao Y, Cai C, Yang H, Zhang X, Liu J, Balk SP, Chen S. Oncotarget. 2016 Jan 12;7(2):1754-64. doi: 10.18632/oncotarget.6434. 10.18632/oncotarget.6434 PubMed 26636645
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