HA-HA-AR-DBD-LBD
(Plasmid #171237)

• Purpose: Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain and Ligand-binding domain (DBD-LBD)
• Depositing Lab: Steven Balk
• Publication: Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. doi: 10.18632/oncotarget.6434.

Backbone

• Vector backbone: pcDNA3.1(+)-kozakHAHA
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 2xHA-tagged human androgen receptor trancate:DNA-binding domain and Ligand-binding domain
• Alt name: HA-HA-AR-DBD-LBD
• Alt name: 2xHA-AR-DBD-LBD
• Species: H. sapiens (human)
• Insert Size (bp): 1200
• Mutation: AR truncate
• Entrez Gene: AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: CMV_fwd_primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For cloning purpose, an "A" nucleotide was inserted right after BamHI site in the vector backbone by DNA mutagensis to change the open reading frame.