pCIneo-AR-S90A
(Plasmid
#171225)
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PurposeMammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser90Ala (AR-S90A)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 171225 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCIneo
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression, Bacterial Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman androgen receptor Ser90Ala mutant
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Alt nameAR-S90A
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SpeciesH. sapiens (human)
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Insert Size (bp)3000
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MutationAR-Ser90Ala (AR-S90A)
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Entrez GeneAR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer CMV_fwd_primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Mutation in an AR Ser-Pro phosphorylation site, and the exact amino acid residue position and composition may slightly vary based on human AR versions.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCIneo-AR-S90A was a gift from Steven Balk (Addgene plasmid # 171225 ; http://n2t.net/addgene:171225 ; RRID:Addgene_171225) -
For your References section:
Androgen receptor phosphorylation and stabilization in prostate cancer by cyclin-dependent kinase 1. Chen S, Xu Y, Yuan X, Bubley GJ, Balk SP. Proc Natl Acad Sci U S A. 2006 Oct 24;103(43):15969-74. doi: 10.1073/pnas.0604193103. Epub 2006 Oct 16. 10.1073/pnas.0604193103 PubMed 17043241