pAAP2
(Plasmid
#171208)
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Purposebacterial expression for purification of PGL-3, allowing cleavage for MBP tag using TEV protease
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 171208 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMAL-c2X
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepgl-3
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SpeciesC. elegans (nematode)
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Entrez Genepgl-3 (a.k.a. CELE_C18G1.4)
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Tag
/ Fusion Protein
- MBP::HIS::TEV cleavage site (N terminal on insert)
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.10.15.340570v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAP2 was a gift from Geraldine Seydoux (Addgene plasmid # 171208 ; http://n2t.net/addgene:171208 ; RRID:Addgene_171208) -
For your References section:
Coordination of RNA and protein condensation by the P granule protein MEG-3. Schmidt H, Putnam A, Rasoloson D, Seydoux G. bioRxiv 2020.10.15.340570 10.1101/2020.10.15.340570