Skip to main content
Addgene

pAAP21
(Plasmid #171207)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171207 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pet28a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    meg-3
  • Species
    C. elegans (nematode)
  • Mutation
    aa545-862, F705A,Y708A,Y713A, N725A
  • Entrez Gene
    meg-3 (a.k.a. CELE_F52D2.4)
  • Tag / Fusion Protein
    • 8xHIS (N terminal on insert)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAP21 was a gift from Geraldine Seydoux (Addgene plasmid # 171207 ; http://n2t.net/addgene:171207 ; RRID:Addgene_171207)

  • For your References section:

    Coordination of RNA and protein condensation by the P granule protein MEG-3. Schmidt H, Putnam A, Rasoloson D, Seydoux G. bioRxiv 2020.10.15.340570 10.1101/2020.10.15.340570
Related items: