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Addgene

pHS33
(Plasmid #171202)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171202 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-6p-1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    meg-3
  • Species
    C. elegans (nematode)
  • Mutation
    aa545-862, F705A,Y708A,Y713A, N725A
  • Entrez Gene
    meg-3 (a.k.a. CELE_F52D2.4)
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHS33 was a gift from Geraldine Seydoux (Addgene plasmid # 171202 ; http://n2t.net/addgene:171202 ; RRID:Addgene_171202)

  • For your References section:

    Coordination of RNA and protein condensation by the P granule protein MEG-3. Schmidt H, Putnam A, Rasoloson D, Seydoux G. bioRxiv 2020.10.15.340570 10.1101/2020.10.15.340570
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