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Addgene

pKAR2-Br512g3.1
(Plasmid #171200)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171200 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKAR2
  • Backbone manufacturer
    In-house made
  • Backbone size w/o insert (bp) 5187
  • Total vector size (bp) 6218
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Engineered Bst DNA Polymerase I Large Fragment
  • Alt name
    Br512g3.1
  • Species
    Synthetic; Geobacillus stearothermophilus
  • Insert Size (bp)
    1932
  • GenBank ID
    4O0I_A
  • Promoter T7
  • Tag / Fusion Protein
    • 8x His tag; Villin Headpiece 47 amino acid (vHP47, supercharged) ; Linker (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer CCTATAAAAATAGGCGTATCACGAGGC
  • 3′ sequencing primer GTGTAACGACCGGCTAGAATCACTCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKAR2-Br512g3.1 was a gift from Andrew Ellington (Addgene plasmid # 171200 ; http://n2t.net/addgene:171200 ; RRID:Addgene_171200)

  • For your References section:

    Portable nucleic acid tests for rapid detection of monkeypox virus. Bhadra S, Ellington AD. medRxiv 2022.08.09.22278605 10.1101/2022.08.09.22278605
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