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pTetR-LasR-pLuxR-GFP
(Plasmid #171159)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171159 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSB1A2
  • Backbone size w/o insert (bp) 2079
  • Total vector size (bp) 3966
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    lasR
  • Alt name
    transcriptional regulator LasR
  • Species
    Pseudomonas aeruginosa
  • Insert Size (bp)
    723
  • Promoter pTet

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site SpeI (destroyed during cloning)
  • 5′ sequencing primer Nil
  • 3′ sequencing primer Nil
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    gfp
  • Alt name
    green fluorescence protein GFP
  • Species
    Aequorea victoria
  • Insert Size (bp)
    717
  • Promoter pLux

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site SpeI (destroyed during cloning)
  • 5′ sequencing primer Nil
  • 3′ sequencing primer Nil
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTetR-LasR-pLuxR-GFP was a gift from Matthew Chang (Addgene plasmid # 171159 ; http://n2t.net/addgene:171159 ; RRID:Addgene_171159)

  • For your References section:

    Engineering microbes to sense and eradicate Pseudomonas aeruginosa, a human pathogen. Saeidi N, Wong CK, Lo TM, Nguyen HX, Ling H, Leong SS, Poh CL, Chang MW. Mol Syst Biol. 2011 Aug 16;7:521. doi: 10.1038/msb.2011.55. 10.1038/msb.2011.55 PubMed 21847113
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