M38 TOP-dGFP
(Plasmid #17114)

• Depositing Lab: Randall Moon
• Publication: Other Plasmids (unpublished)

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4000

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Tcf/Lef sites
• Alt name: beta catenin reporter
• Insert Size (bp): 1000
• Entrez Gene: Ctnnb1 (a.k.a. Bfc, Catnb, Mesc)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SP6
• 3′ sequencing primer: EBV rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The promoter was from Hans Clevers' construct, TOPFLASH.
• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Description: This is a destabilized GFP under the control of a beta-catenin responsive promoter.

A 358-bp promoter/enhancer region of TOPFLASH, containing 4 consensus Lef binding sites and a minimal promoter, was amplified by PCR. The 94-bp minimal promoter is derived from the mouse cFos gene (Accession No. AF332140), starting 38 bp 5.

The full sequence provided by the depositing lab was created by piecing together various sequences, and is not exact. It should be used as a guide only.

TCF/LEF optimal sequence - cctttgatcttacc