pCI-Flag-NEDD4L∆WW1,2,4
(Plasmid #171137)

• Purpose: expresses NEDD4L with inactivating mutation in first, second and fourth WW domain, but intact third WW domain, in mammalian cells
• Depositing Lab: Wesley Sundquist
• Publication: Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10.1016/j.jbc.2021.100975.

Backbone

• Vector backbone: pCI-FLAG
• Backbone size w/o insert (bp): 5842
• Total vector size (bp): 8047
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: NEDD4L
• Species: H. sapiens (human)
• Insert Size (bp): 2565
• Mutation: changed tryptophan 221 to alanine, tryptophan 453 to alanine, tryptophan 576 to alanine
• Entrez Gene: NEDD4L (a.k.a. NEDD4-2, NEDD4.2, PVNH7, RSP5, hNEDD4-2)
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TAATACGACTCACTATAGG
• 3′ sequencing primer: GCATTAACCCTCACTAAAG

Resource Information

• Supplemental Documents:
  • pCI-Nedd4l(iso2)deltaWW1,2,4.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry