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pmirGlo-RBM8A-3'UTRAluY-Sz mut 2598-4066
(Plasmid #171135)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171135 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmirGlo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 7350
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RBM8A 3'UTR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1467
  • Mutation
    A3302G, A3356G, A3373G, A3389G,A3451G, T3729C, A3733G, A3739G, A3792G, A3806G, A3861G, A3887G refering to NM_005105.5
  • Entrez Gene
    RBM8A (a.k.a. BOV-1A, BOV-1B, BOV-1C, C1DELq21.1, DEL1q21.1, MDS014, RBM8, RBM8B, TAR, Y14, ZNRP, ZRNP1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 3′ sequencing primer T7 term
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmirGlo-RBM8A-3'UTRAluY-Sz mut 2598-4066 was a gift from Emanuela Felley-Bosco (Addgene plasmid # 171135 ; http://n2t.net/addgene:171135 ; RRID:Addgene_171135)

  • For your References section:

    Double-Stranded RNA Structural Elements Holding the Key to Translational Regulation in Cancer: The Case of Editing in RNA-Binding Motif Protein 8A. Abukar A, Wipplinger M, Hariharan A, Sun S, Ronner M, Sculco M, Okonska A, Kresoja-Rakic J, Rehrauer H, Qi W, Beusechem VWV, Felley-Bosco E. Cells. 2021 Dec 15;10(12). pii: cells10123543. doi: 10.3390/cells10123543. 10.3390/cells10123543 PubMed 34944051
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