pmirGlo-RBM8A-3'UTR AluSz 3501-4065
(Plasmid
#171132)
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Purposereporter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 171132 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmirGlo
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 7350
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRBM8A 3'UTR
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SpeciesH. sapiens (human)
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Insert Size (bp)564
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Entrez GeneRBM8A (a.k.a. BOV-1A, BOV-1B, BOV-1C, C1DELq21.1, DEL1q21.1, MDS014, RBM8, RBM8B, TAR, Y14, ZNRP, ZRNP1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 3′ sequencing primer T7 term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmirGlo-RBM8A-3'UTR AluSz 3501-4065 was a gift from Emanuela Felley-Bosco (Addgene plasmid # 171132 ; http://n2t.net/addgene:171132 ; RRID:Addgene_171132) -
For your References section:
Double-Stranded RNA Structural Elements Holding the Key to Translational Regulation in Cancer: The Case of Editing in RNA-Binding Motif Protein 8A. Abukar A, Wipplinger M, Hariharan A, Sun S, Ronner M, Sculco M, Okonska A, Kresoja-Rakic J, Rehrauer H, Qi W, Beusechem VWV, Felley-Bosco E. Cells. 2021 Dec 15;10(12). pii: cells10123543. doi: 10.3390/cells10123543. 10.3390/cells10123543 PubMed 34944051