Skip to main content
Addgene

pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)
(Plasmid #171129)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171129 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pUC19-U6 (derivative of BPK1520)
  • Modifications to backbone
    Cloned into BsmBI + HindIII digested BPK1520 (Addgene ID 65777)
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    U6 promoter entry plasmid for LwaCas13a crRNA cloning
  • Alt name
    LTH151
  • gRNA/shRNA sequence
    entry plasmid
  • Species
    H. sapiens (human)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer os280 (5'-CAGGGTTATTGTCTCATGAGCGG-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151) was a gift from Benjamin Kleinstiver (Addgene plasmid # 171129 ; http://n2t.net/addgene:171129 ; RRID:Addgene_171129)

  • For your References section:

    Lack of Cas13a inhibition by anti-CRISPR proteins from Leptotrichia prophages. Johnson MC, Hille LT, Kleinstiver BP, Meeske AJ, Bondy-Denomy J. Mol Cell. 2022 Jun 2;82(11):2161-2166.e3. doi: 10.1016/j.molcel.2022.05.002. Epub 2022 May 26. 10.1016/j.molcel.2022.05.002 PubMed 35623354
Related items:
Commonly requested with: