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pCMV-T7-null (BPK1077)
(Plasmid #171128)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171128 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    JDS246 (Addgene ID 43861)
  • Vector type
    Mammalian Expression, Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pCMV-T7 null expression plasmid that encodes a two amino acid peptide (Met-Asp)
  • Promoter pCMV and T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer oBK6928 (5'-CCAAGTCTCCACCCCATTGACG-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-T7-null (BPK1077) was a gift from Benjamin Kleinstiver (Addgene plasmid # 171128 ; http://n2t.net/addgene:171128 ; RRID:Addgene_171128)

  • For your References section:

    Lack of Cas13a inhibition by anti-CRISPR proteins from Leptotrichia prophages. Johnson MC, Hille LT, Kleinstiver BP, Meeske AJ, Bondy-Denomy J. Mol Cell. 2022 Jun 2;82(11):2161-2166.e3. doi: 10.1016/j.molcel.2022.05.002. Epub 2022 May 26. 10.1016/j.molcel.2022.05.002 PubMed 35623354
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