pDB4695
(Plasmid
#171127)
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PurposeA plasmid containing the codon-optimized OsTIR1-F74A, which is under the control of adh1 promoter. It served as one of the F-box protein expression plasmids in AID system.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 171127 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDB4697
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Vector typeOther
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOsTIR1-F74A
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SpeciesOther
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Mutationnon applicable
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.07.19.452993v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDB4695 was a gift from Li-Lin Du (Addgene plasmid # 171127 ; http://n2t.net/addgene:171127 ; RRID:Addgene_171127) -
For your References section:
An improved auxin-inducible degron system for fission yeast. Zhang XR, Zhao L, Suo F, Gao Y, Wu Q, Qi X, Du LL. G3 (Bethesda). 2022 Jan 4;12(1):jkab393. doi: 10.1093/g3journal/jkab393. 10.1093/g3journal/jkab393 PubMed 34849776
