pDB4697
(Plasmid
#171125)
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PurposeA plasmid containing the ura4 genomic region,which flanked by a NotI site at the both ends. It served as the control plasmid or backbone for the ura4 integrated plasmid construction.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 171125 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEASY-blunt
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Backbone manufacturerTransGen Biotech
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Vector typeOther
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameura4
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SpeciesS. pombe (fission yeast)
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Mutationnon applicable
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.07.19.452993v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDB4697 was a gift from Li-Lin Du (Addgene plasmid # 171125 ; http://n2t.net/addgene:171125 ; RRID:Addgene_171125) -
For your References section:
An improved auxin-inducible degron system for fission yeast. Zhang XR, Zhao L, Suo F, Gao Y, Wu Q, Qi X, Du LL. G3 (Bethesda). 2022 Jan 4;12(1):jkab393. doi: 10.1093/g3journal/jkab393. 10.1093/g3journal/jkab393 PubMed 34849776