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PurposeA kanMX-marked pFA6a based plasmid, which has a 7-aa linker, a 16-aa XTEN16 linker, three tandam repeats of sAID. It serves as the template for 3_sAID degron tagging at the C-terminus.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 171124 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFA6a-kanMX6
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Vector typeOther
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3_sAID
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SpeciesA. thaliana (mustard weed)
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Mutationnon applicable
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.07.19.452993v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDB4581 was a gift from Li-Lin Du (Addgene plasmid # 171124 ; http://n2t.net/addgene:171124 ; RRID:Addgene_171124) -
For your References section:
An improved auxin-inducible degron system for fission yeast. Zhang XR, Zhao L, Suo F, Gao Y, Wu Q, Qi X, Du LL. G3 (Bethesda). 2022 Jan 4;12(1):jkab393. doi: 10.1093/g3journal/jkab393. 10.1093/g3journal/jkab393 PubMed 34849776
