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pLVX-TetOne-Puro-p21
(Plasmid #171122)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171122 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX-TetOne-Puro
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 9175
  • Total vector size (bp) 9670
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    p21
  • Alt name
    CDKN1A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    495
  • Entrez Gene
    CDKN1A (a.k.a. CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pLVX-TetOne-Puro backbone from pLVX-TetOne-Puro-hAXL (Plasmid #124797)
p21 insert from pcDNA3-HA p21 (Plasmid #78782)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-TetOne-Puro-p21 was a gift from Jason Sheltzer (Addgene plasmid # 171122 ; http://n2t.net/addgene:171122 ; RRID:Addgene_171122)

  • For your References section:

    Chromosomal instability accelerates the evolution of resistance to anti-cancer therapies. Lukow DA, Sausville EL, Suri P, Chunduri NK, Wieland A, Leu J, Smith JC, Girish V, Kumar AA, Kendall J, Wang Z, Storchova Z, Sheltzer JM. Dev Cell. 2021 Sep 13;56(17):2427-2439.e4. doi: 10.1016/j.devcel.2021.07.009. Epub 2021 Aug 4. 10.1016/j.devcel.2021.07.009 PubMed 34352222
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