pAAV gfaABC1D 2xLyn-ERex-Venus-bPAC(F198Y)
(Plasmid #171117)

• Purpose: Astrocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity
• Depositing Lab: Thomas Oertner
• Publication: Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10.1186/s12915-021-01151-9.

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Backbone

• Vector backbone: pAAV
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 5055
• Total vector size (bp): 6941
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 2xLyn-ERex-Venus-bPAC(F198Y)
• Alt name: PACmn
• Species: Synthetic; Beggiatoa
• Insert Size (bp): 1885
• Promoter: gfaABC1D
• Tag / Fusion Protein:
  • Venus

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: GTTGATTATCGATAAGCTTA
• 3′ sequencing primer: GTAATCCAGAGGTTGATTATCG

Resource Information

• Supplemental Documents:
  • pAAV-gfaABC1D-Lyn11-ERex-Venus-b PAC F198Y.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert was assembled in the lab of Georg Nagel, the promotor is from Baljit Khakh
Addgene ID #52925