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Addgene

3xFlag-HaloTag-EZH2 HDR - pDY053
(Plasmid #171108)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171108 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    puc19
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EZH2
  • Species
    H. sapiens (human)
  • Entrez Gene
    EZH2 (a.k.a. ENX-1, ENX1, EZH2b, KMT6, KMT6A, WVS, WVS2)
  • Tag / Fusion Protein
    • HaloTag (N terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3xFlag-HaloTag-EZH2 HDR - pDY053 was a gift from Thomas Cech (Addgene plasmid # 171108 ; http://n2t.net/addgene:171108 ; RRID:Addgene_171108)

  • For your References section:

    Live-cell imaging reveals the dynamics of PRC2 and recruitment to chromatin by SUZ12-associated subunits. Youmans DT, Schmidt JC, Cech TR. Genes Dev. 2018 Jun 1;32(11-12):794-805. doi: 10.1101/gad.311936.118. Epub 2018 Jun 11. 10.1101/gad.311936.118 PubMed 29891558
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