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pTO128_pPZP-NATca
(Plasmid #171105)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171105 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPZP-NEO
  • Backbone size w/o insert (bp) 9309
  • Total vector size (bp) 10522
  • Vector type
    A. tumefaciens TI Plasmid
  • Selectable markers
    Nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NAT (Nourseothricin Resistance)
  • Species
    C. auris
  • Insert Size (bp)
    1213
  • Promoter AgTEF2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CAGGAAACAGCTATGAC
  • 3′ sequencing primer GTAAAACGACGGCCAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTO128_pPZP-NATca was a gift from Teresa O'Meara (Addgene plasmid # 171105 ; http://n2t.net/addgene:171105 ; RRID:Addgene_171105)

  • For your References section:

    Forward and reverse genetic dissection of morphogenesis identifies filament-competent Candida auris strains. Santana DJ, O'Meara TR. Nat Commun. 2021 Dec 10;12(1):7197. doi: 10.1038/s41467-021-27545-5. 10.1038/s41467-021-27545-5 PubMed 34893621
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