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pTO136_pADH1-tRNA-Ala-gRNA-tracrRNA-HDV-tAgTEF2
(Plasmid #171104)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171104 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2683
  • Total vector size (bp) 4075
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    sgRNA
  • Species
    C. auris
  • Insert Size (bp)
    1392
  • Promoter ADH1

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTAAAACGACGGCCAG
  • 3′ sequencing primer CAGGAAACAGCTATGAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTO136_pADH1-tRNA-Ala-gRNA-tracrRNA-HDV-tAgTEF2 was a gift from Teresa O'Meara (Addgene plasmid # 171104 ; http://n2t.net/addgene:171104 ; RRID:Addgene_171104)

  • For your References section:

    Forward and reverse genetic dissection of morphogenesis identifies filament-competent Candida auris strains. Santana DJ, O'Meara TR. Nat Commun. 2021 Dec 10;12(1):7197. doi: 10.1038/s41467-021-27545-5. 10.1038/s41467-021-27545-5 PubMed 34893621
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