V19 pCD-beta g FLT
(Plasmid
#17110)
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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17110 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCD-Bg FLT
- Backbone size (bp) 4200
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
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Tags
/ Fusion Proteins
- beta-globin 5' UTR (N terminal on backbone)
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Notes for Use:
Constructed by: Julia Yang-Snyder.
SV40 ori/early promoter driving expression of beta-globin 5' UTR and.
amino terminal flag-tagged fusion proteins. (reading frame same as pCS3+)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
V19 pCD-beta g FLT was a gift from Randall Moon (Addgene plasmid # 17110 ; http://n2t.net/addgene:17110 ; RRID:Addgene_17110)