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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Ascl1 x2)
(Plasmid #171099)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171099 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PX458
  • Backbone manufacturer
    Feng Zhang (Addgene plasmid # 48138)
  • Vector type
    CRISPR
  • Selectable markers
    mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hSpCas9
  • gRNA/shRNA sequence
    Ascl1 gRNA1: AGCCTGCTTCTTTGCGACCg; Ascl1 gRNA2: AGGGGGCGGTCACAAGTCAG
  • Species
    M. musculus (mouse); S. pyogenes
  • Entrez Gene
    Ascl1 (a.k.a. ASH1, Mash1, bHLHa46)
  • Promoter Ef1a

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Ascl1 x2) was a gift from Joseph Brzezinski (Addgene plasmid # 171099 ; http://n2t.net/addgene:171099 ; RRID:Addgene_171099)

  • For your References section:

    Initiation of Otx2 expression in the developing mouse retina requires a unique enhancer and either Ascl1 or Neurog2 activity. Kaufman ML, Goodson NB, Park KU, Schwanke M, Office E, Schneider SR, Abraham J, Hensley A, Jones KL, Brzezinski JA. Development. 2021 Jun 15;148(12). pii: 269198. doi: 10.1242/dev.199399. Epub 2021 Jun 18. 10.1242/dev.199399 PubMed 34143204
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