pQ-NFY-A-GB-HC
(Plasmid #171081)

• Purpose: Co-expresses of NFY complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding the three subunit NF-Y, with untagged NFYA, GST-NFYB, and 6-His-NFYC
• Depositing Lab: Joan Conaway
• Publication: He et al Proc Natl Acad Sci U S A. 2021 Sep 28;118(39):e2108751118. doi: 10.1073/pnas.2108751118.

Backbone

• Vector backbone: pQE-2
• Total vector size (bp): 8787
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: NF-YA
• Alt name: NF-YA
• Species: H. sapiens (human)
• Insert Size (bp): 957
• GenBank ID: NM_021705.3
• Entrez Gene: NFYA (a.k.a. CBF-A, CBF-B, HAP2, NF-YA)
• Promoter: T5 promoter

Cloning Information for Gene/Insert 1

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: TGAGCGGATAACAATTTCACACAG
• 3′ sequencing primer: GGCAACCGAGCGTTCTGAAC

Gene/Insert 2

• Gene/Insert name: NF-YB
• Species: H. sapiens (human)
• Insert Size (bp): 1326
• GenBank ID: NM_006166.3
• Entrez Gene: NFYB (a.k.a. CBF-A, CBF-B, HAP3, NF-YB)
• Promoter: T5 promoter
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information for Gene/Insert 2

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: TGAGCGGATAACAATTTCACACAG
• 3′ sequencing primer: GGCAACCGAGCGTTCTGAAC

Gene/Insert 3

• Gene/Insert name: NF-YC
• Species: H. sapiens (human)
• Insert Size (bp): 1068
• GenBank ID: NM_014223.4
• Entrez Gene: NFYC (a.k.a. CBF-C, CBFC, H1TF2A, HAP5, HSM, NF-YC)
• Promoter: T5 promoter
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information for Gene/Insert 3

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: TGAGCGGATAACAATTTCACACAG
• 3′ sequencing primer: GGCAACCGAGCGTTCTGAAC

Resource Information

• Supplemental Documents:
  • pQLink- NFY-ABC.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry