pmRuby2-CrowdingLinker-Clover
(Plasmid #171058)

• Purpose: Expresses tandem mClover2-mRuby2 FRET pair with a crowdedness-sensitve linker domain to sense cytosolic crowdedness.
• Depositing Lab: Ke Xu
• Publication: Chen et al Light Sci Appl. 2021 May 8;10(1):97. doi: 10.1038/s41377-021-00536-3.

Backbone

• Vector backbone: pClover-mRuby2-FRET-10
• Backbone manufacturer: Michael Davidson
• Backbone size w/o insert (bp): 5395
• Total vector size (bp): 5758
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Crowdedness-sensitive linker domain
• Species: Synthetic
• Insert Size (bp): 300
• Promoter: CMV
• Tags / Fusion Proteins:
  • mRuby2 (N terminal on insert)
  • mClover2 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • pmRuby2-CrowdingLinker-Clover.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry