pHR LAT/Zap70 iLID-Drop
(Plasmid
#171038)
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PurposeLight-induced LAT-Zap70 dimerization and LAT clustering. Zap70 is TagRFP-labeled.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 171038 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHR
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Vector typeMammalian Expression, Lentiviral
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Selectable markersTagRFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLAT
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SpeciesH. sapiens (human)
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Entrez GeneLAT (a.k.a. IMD52, LAT1, pp36)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHR LAT/Zap70 iLID-Drop was a gift from Jared Toettcher (Addgene plasmid # 171038 ; http://n2t.net/addgene:171038 ; RRID:Addgene_171038) -
For your References section:
Positive feedback between the T cell kinase Zap70 and its substrate LAT acts as a clustering-dependent signaling switch. Dine E, Reed EH, Toettcher JE. Cell Rep. 2021 Jun 22;35(12):109280. doi: 10.1016/j.celrep.2021.109280. 10.1016/j.celrep.2021.109280 PubMed 34161759